The objective of this work was to establish a cell micropatterning method that warrants reproducible cell shape, enabling a fast and accurate quantification of cytoskeletal organization. In order to achieve this goal, following major issues were addressed:
-Micropatterns with different shapes and sizes were designed and fabricated.
-µCP and stencil patterning were tested and compared in regard to their performance in fabricating micropatterns and in printing a uniform protein coating.
-The behavior of two selected cell lines was observed on micropatterned substrates.
-The optimal micropattern size and shape for a high yield of single cells was detected.
-The KIF distribution was assessed in normalized cells.
-Heat maps were generated for normalized cells.